New two-photon calcium microscope records Firing of Thousands of Individual Neurons in 3-D
UCLA neuroscientists have collaborated with physicists to develop a non-invasive, ultra-high-speed microscope that can record in real time the firing of thousands of individual neurons in the brain as they communicate, or miscommunicate, with each other.
Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing
Adrian Cheng, J Tiago Gonçalves, Peyman Golshani, Katsushi Arisaka & Carlos Portera-Cailliau
Nature Methods (2011) doi:10.1038/nmeth.1552
Received 01 October 2010 Accepted 14 December 2010 Published online 09 January 2011
In vivo two-photon calcium imaging would benefit from the use of multiple excitation beams to increase scanning speed, signal-to-noise ratio and field of view or to image different axial planes simultaneously. Using spatiotemporal multiplexing we circumvented light-scattering ambiguity inherent to deep-tissue multifocal two-photon microscopy. We demonstrate calcium imaging at multiple axial planes in the intact mouse brain to monitor network activity of ensembles of cortical neurons in three spatial dimensions.